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育亨賓對(duì)異丙酚麻醉中大鼠海馬去甲基腎上腺素釋放的影響
畢業(yè)論文摘要:
目的:觀察大鼠異丙酚麻醉與中樞神經(jīng)系統(tǒng)海馬去甲基腎上腺素(NA)的釋放以及α2-NA受體抑制劑之間的相互關(guān)系,探討中樞性NA機(jī)制在異丙酚麻醉中發(fā)生的作用。
方法:選擇20只雄性SD大鼠,隨機(jī)分為A,B2組,每組10只。A組:微量泵以10mg/kg/h,60mg/kg/h的速度依次各靜注45min,停止靜注異丙酚直至動(dòng)物清醒。B組在輸注異丙酚的同時(shí)腹腔內(nèi)注射育亨賓0.5mg/kg,余和A組相同。采用微透析技術(shù)分別測(cè)定異丙酚麻醉前、中及蘇醒期的大鼠海馬區(qū)細(xì)胞外液的NA釋放值。
結(jié)果:A組和B組,NA的釋放在整個(gè)麻醉過(guò)程中的變化趨勢(shì)有顯著差異(P<0.05)。其中B組的NA的釋放量在整個(gè)麻醉過(guò)程中的變化波動(dòng)較大并呈升高的趨勢(shì),最高為(0.19±0.02)μg/15min,最低為(0.13±0.03)μg/15min;而A組NA的釋放量在整個(gè)麻醉過(guò)程中的變化則較平穩(wěn),最高為(0.16±0.01)μg/15min,最低(0.14±0.02)μg/15min。
結(jié)論:異丙酚麻醉抑制海馬區(qū)NA的釋放,育亨賓可使此作用減弱。
關(guān)鍵詞:海馬;腦;微透析;異丙酚;育亨賓;去甲基腎上腺素;大鼠
本文作者:賀賽琳,張英民,岳 云,陳小光,郭 徽,劉 蘇
Effects of propofol anesthesia on noradrenaline release in rat hippocampus
賀賽琳1,張英民1,岳 云2,陳小光1,郭 徽1,劉 蘇1
(1解放軍304醫(yī)院麻醉科,北京100037,2首都醫(yī)科大學(xué)附屬紅10字朝陽(yáng)醫(yī)院麻醉科,北京100021)
HE Sai-Lin1, ZHANG Ying-Min1, YUE Yun2, CHEN Xiao-Guang1, GUO Hui1, LIU Su1
1Department of Anesthesiology, 304th Hospital of Chinese PLA, Beijing 100037, China, 2Department of Anesthesiology, Chaoyang Hospital, Capital Medical Science University, Beijing 100021, China
Abstract
Aim: To observe the influence s of propofol anesthesia on the release of noradrenaline (NA) andα2-NA receptorinhibitor yohimbine in rat hippocampus so as to identify themechanisms of central noradrenaline in propofol anesthesia.
Methods: Twenty adult male SD rats were divided randomly into 2 groups. In group A , the rats were infused intravenously(IV) with propofol at a rate of 10 mg/kg/h for 45 min and 60mg/kg/h for 45 min and in group B , the rats were givenyohimbine 0.5 mg/kg intraperitoneally and were infused IV withpropofol a s did group A. The release of NA in the extra-cellularliquid of hippocampus was quantified before propofol anesthesia, in propofol administration and after propofol administrationby in vivo microdialysis technique.
Results: Compared with those of the group A, the NA levels in hippocampus of group Bchanged significantly during the whole anesthesia course , with the highest release being(0.19±0.02)μg/15min and the lowest release being(0.13±0.03)μg/15min. The NA levelsin group A changed steadily [from(0.16±0.01)μg/15min to(0.14±0.02)μg/15min], (P<0.05).
Conclusion: Propofol anesthesia can inhibit the NA release in hippocampus but yohimbine increases NA release in hippocampus in propofol anesthesia.
Keywords: hippocampus; brain; microdialysis; propofol; yohimbine; noradrenaline; rat
0 引言
去甲基腎上腺素(NA)是中樞神經(jīng)系統(tǒng)(CNS)重要的神經(jīng)遞質(zhì),測(cè)定某腦區(qū)NA釋放的變化可以反映腦內(nèi)單胺類神經(jīng)遞質(zhì)代謝的狀態(tài)。α2-NA受體在大腦皮層、腦干的藍(lán)斑核(LC)、海馬、孤束核等處分布的密度很高。其生理功能與維持CNS覺醒有關(guān),可能與全麻藥中樞作用機(jī)制有1定聯(lián)系。我們應(yīng)用腦內(nèi)微透析技術(shù),觀察大鼠異丙酚麻醉及其在NA特定拮抗劑作用下與CNS海馬NA的釋放以及α2-NA受體之間的相互關(guān)系如下。
摘要:
目的:觀察大鼠異丙酚麻醉與中樞神經(jīng)系統(tǒng)海馬去甲基腎上腺素(NA)的釋放以及α2-NA受體抑制劑之間的相互關(guān)系,探討中樞性NA機(jī)制在異丙酚麻醉中發(fā)生的作用。
方法:選擇20只雄性SD大鼠,隨機(jī)分為A,B2組,每組10只。A組:微量泵以10mg/kg/h,60mg/kg/h的速度依次各靜注45min,停止靜注異丙酚直至動(dòng)物清醒。B組在輸注異丙酚的同時(shí)腹腔內(nèi)注射育亨賓0.5mg/kg,余和A組相同。采用微透析技術(shù)分別測(cè)定異丙酚麻醉前、中及蘇醒期的大鼠海馬區(qū)細(xì)胞外液的NA釋放值。
結(jié)果:A組和B組,NA的釋放在整個(gè)麻醉過(guò)程中的變化趨勢(shì)有顯著差異(P<0.05)。其中B組的NA的釋放量在整個(gè)麻醉過(guò)程中的變化波動(dòng)較大并呈升高的趨勢(shì),最高為(0.19±0.02)μg/15min,最低為(0.13±0.03)μg/15min;而A組NA的釋放量在整個(gè)麻醉過(guò)程中的變化則較平穩(wěn),最高為(0.16±0.01)μg/15min,最低(0.14±0.02)μg/15min。
結(jié)論:異丙酚麻醉抑制海馬區(qū)NA的釋放,育亨賓可使此作用減弱。
關(guān)鍵詞:海馬;腦;微透析;異丙酚;育亨賓;去甲基腎上腺素;大鼠
本文作者:賀賽琳,張英民,岳 云,陳小光,
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【育亨賓對(duì)異丙酚麻醉中大鼠海馬去甲基腎上腺素釋放的影響】相關(guān)文章:
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